Our lab has recently developed a quantitative method for the analysis of cellular shape and spatial characteristics of bacteria. Seen above is the algorithmic application of our cell-segmenting and masking to a snapshot of cells imaged using a confocal microscope. The above cells are then individually enumerated and quantitatively analyzed to determine several cellular and nucleoid-based vectors. This data is an important factor in emerging new biology, wherein we are attempting to regulate genes by utilizing naturally occurring molecular condensates under stress and influencing the expression of nucleoid associated proteins in Deinococcus radiodurans. Contributors: Dr. Lydia Contreras, Antonio Cordova, Brandon Niese, Dr. Vernita Gordon