The development of ELISA is based on the immobilization and enzyme labeling of antigen or antibody. The immunological activity of antigen or antibody remains after the immobilization. And enzyme-labeled antigen or antibody remains both their immunological activity and enzymatic activity.
Materials: Antigen Serum
Reagent and kit: Carbonate coating buffer PBST BSA
Equipment and Supplies: 96-Well ELISA plates 4℃refrigerator Incubator Spectrophotometer
ELISA used in Creative BioMart:
Enzyme/Antibody conjugation Host Cell Protein Mitigation
Epitope Mapping Pharmaceutical Stability Analysis
Biopharmaceutical Process and Product Related Impurities Analysis
Source: http://www.creativebiomart.net/blog/principle-and-protocol-of-enzyme-linked-immunosorbent-assay-elisa/